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Peptide Applications Overview

Peptides can be used in a wide variety of research applications:

Immunology

Immune Monitoring

The tracking of immune responses after a particular drug treatment or immunization is a useful way of monitoring the effectiveness of treatments.  There are numerous methods used for the purposes of monitoring immune responses.

     ELISpot

     Intracellular Cytokine Staining

     Cytotoxic T Lymphocyte Killing Assays

     Proliferation Assays

CFSE

3H Thymidine

Epitope Discovery

The aim of epitope mapping projects is to identify and characterize novel epitopes from a protein that are recognized by the immune system.   By identifying novel epitopes, strategies can be developed to produce immunotherapies or vaccines for a wide range of disease areas such as cancers or infectious diseases.

T cell Epitope Discovery

     ELISpot

     Intracellular Cytokine Staining

     Proliferation Assays

     MHC Peptide Binding Assays

     B Cell Epitope Mapping

ELISA

Inhibition Assays

Cell Signaling

The phosphorylation/dephosphorylation process is regulated by kinases and phosphatases respectively.  Many hundreds of these individual enzymes have been identified and disruption to any one of them can have multiple effects on downstream pathways.  A variety of disease pathologies, particularly cancer, have been associated with mutations or deletions in signalling pathways, making the identification of new kinases and phosphatases, and also their inhibitors, an important target for many research projects.

Kinase Assays

Radio-labeled Kinase Assay

ELISA Based Assay

Fluorescent Based Assay

Fluorescence Polarization Assay

Fluorescence Resonance Energy Transfer (FRET)

Time Resolved Fluorescence (TRF)

Mobility Shift Assays

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